Comparison of bacterial genome assembly software for MinION data
نویسندگان
چکیده
I O N d a t a. O u r o b j e c t i v e w a s t o c o m p a r e a n d c o n t r a s t t h e a c c u r a c y a n d c h a r a c t 0 0 a n d t h e s h o r t r e a d d a t a w a s. CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not .
منابع مشابه
Comparison of bacterial genome assembly software for MinION data and their applicability to medical microbiology
Translating the Oxford Nanopore MinION sequencing technology into medical microbiology requires on-going analysis that keeps pace with technological improvements to the instrument and release of associated analysis software. Here, we use a multidrug-resistant Enterobacter kobei isolate as a model organism to compare open source software for the assembly of genome data, and relate this to the ti...
متن کاملThe use of Oxford Nanopore native barcoding for complete genome assembly
Background The Oxford Nanopore Technologies MinION(TM) is a mobile DNA sequencer that can produce long read sequences with a short turn-around time. Here we report the first demonstration of single contig genome assembly using Oxford Nanopore native barcoding when applied to a multiplexed library of 12 samples and combined with existing Illumina short read data. This paves the way for the closu...
متن کاملA single chromosome assembly of Bacteroides fragilis strain BE1 from Illumina and MinION nanopore sequencing data
BACKGROUND Second and third generation sequencing technologies have revolutionised bacterial genomics. Short-read Illumina reads result in cheap but fragmented assemblies, whereas longer reads are more expensive but result in more complete genomes. The Oxford Nanopore MinION device is a revolutionary mobile sequencer that can produce thousands of long, single molecule reads. RESULTS We sequen...
متن کاملA reference bacterial genome dataset generated on the MinION™ portable single-molecule nanopore sequencer
BACKGROUND The MinION™ is a new, portable single-molecule sequencer developed by Oxford Nanopore Technologies. It measures four inches in length and is powered from the USB 3.0 port of a laptop computer. The MinION™ measures the change in current resulting from DNA strands interacting with a charged protein nanopore. These measurements can then be used to deduce the underlying nucleotide sequen...
متن کاملComplete assembly of novel environmental bacterial genomes by MinION sequencing
In this study, we adapt a protocol for the growth of previously uncultured environmental bacterial isolates, to make it compatible with whole genome sequencing. We demonstrate that in combination with the MinION sequencing device, complete assemblies can be derived, allowing genomic comparisons to be made. This approach allows rapid, inexpensive and straightforward discovery, and genomic analys...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016